The prevalence of non-alcoholic fatty liver disease (NAFLD) is considerably higher than previously estimated and is continuing to increase at an alarming rate. It is estimated that almost 5% of adults in the US and Western world have non-alcoholic steatohepatitis (NASH). Given the lack of evidence-based effective therapies for NASH, there is a marked need for diet-induced animal models that better recapitulate cardinal features of the disease. The plethora of currently available diet-induced models to drive the different stages of NAFLD adds an additional challenge when designing these studies. In this webinar, we will review some of the commonly used diets to drive NAFLD in mouse models with focus on their ability to develop advanced stages of NASH and hepatocellular carcinoma (HCC) while maintaining their relevance to human disease. To better assist with model selection, we will briefly describe some of overlooked and understudied aspects of these pre-clinical models including age of the animal, sex differences, control diets, and housing temperature.
Key takeaways from this webinar include:
1. The advantages of purified diets and how they can be used to induce NAFLD, from steatosis to HCC
2. Review the differences between the diets that are commonly used to induce NAFLD: MCD, CDAA, CDAHFD, CDHFD, and HFC/mAMLN/GAN
3. Other considerations- choice of control diet, age and sex differences and thermoneutrality