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Application of high-throughput sequencing technology in agriculture research
Recent advancement in genomic technologies including a remarkable improvement in sequencing throughput and an overall reduction in sequencing cost enables a wider range of applications in agricultural and environmental research areas. Our work focuses primarily on utilizing next-generation sequencing technologies to identify molecular markers linked to important agronomic traits in Thailand’s economic species. We utilized a rapid and economical genotyping-by-sequencing (GBS) approach to efficiently identify single nucleotide polymorphisms (SNPs) for high-density linkage map construction and quantitative trait loci (QTL) mapping. Besides their application in marker-assisted selection, GBS-based SNP markers are useful for studying genetic diversity and population structure as well as for cultivar identification and F1 hybrid seed purity test.


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Dr. Wirulda Nik Pootakham
@National Omics (NOC), National Science and Technology Development Agency (NSTDA), Thailand
Wirulda (Nik) Pootakham studied Plant Molecular Biology at Cornell University and graduated summa cum laude in 2002. She went on to pursue a PhD in Cell and Molecular Biology at Stanford University, studying a signal transduction pathway that regulates nutrient starvation responses in Chlamydomonas, a single-cell green alga. After obtaining a doctorate degree, she joined the Genomic Research Lab at the National Science and Technology Development Agency (NSTDA) with a determination to help drive agricultural sciences in Thailand forward. Her work focuses primarily on applying next-generation sequencing technologies to help identify molecular markers that are linked to important agronomic traits in a number of Thailand’s economic species (rice, cassava, oil palm, rubber tree and sugarcane). Nik also has been involved in projects that address environmental issues of coral bleaching in the Gulf of Thailand and Andaman Sea.