ISOCHORIC PROCESSES IN CRYOBIOLOGY
Boris Rubinsky, Professor of the Graduate School - University of California Berkeley, Department of Mechanical Engineering and Department of Bioengineering, USA
Most of the research in cryobiology was done under conditions of constant atmospheric pressure (isobaric) because this is the normal mode at which life processes take place on earth. We began to explore processes relevant to cryobiology under conditions of constant volume (isochoric). Our theoretical and experimental research has shown that isochoric condition modify cryobiology relevant processes. We studied the effects of isochoric constrains on the phenomena of: supercooling, freezing and vitrification. This presentation is a brief review of the theoretical and experimental findings.
AVOIDING DEVITRIFICATION AFTER CRYOPRESERVATION: HIGH INTENSITY FOCUSED ULTRASOUND
Laura Encabo Luque, Graduate Student - Engineering School, University of Seville (Energy, Chemical and Environmental Engineering), Spain
When a biological sample is cryopreserved, the problem of recrystallization can arises during rewarming. However, if it carries out fast and homogeneous heating this problem is avoided. From CryoBioTech we can offer the solution: by using HIFU as a rewarming technique, the recrystallization is solved. In this work, the survival rate obtained in Caenorhabditis elegans model is presented a proof of this scalable technique.