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DCVMN Webinar: Novel Sf9 Rhabdovirus-Negative Cell Line & Chemically Defined Media Platform
As the industry is trying to walk away from the egg-based vaccine and mammalian cell line production processes, the baculovirus-insect cell (BIC) system is a viable alternative due to its flexibility, speed, simplicity, and eukaryotic protein process capacity. BIC system is widely used to produce recombinant proteins, virus-like particles (VLPs), protein subunit vaccines, and viral vectors, with Spodoptera frugiperda (Sf) cell lines as hosts. However, Sf cell lines contain a Sf-rhabdovirus, which is considered a process contaminant and must be eliminated during the process. To improve the safety profile and reduce lot-to-lot reliability of the production method using the BIC system, we developed a Sf9 rhabdovirus-negative (Sf-RVN®) cell line with companion chemically defined medium. In this webinar, we will demonstrate how the Sf-RVN® cell line and EX-CELL® CD Insect Medium Platform can enhance risk mitigation and enable a safe and efficient biologics production with high productivity.
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