Dr. Dominika Czaplinska & Dr. Roxane Crouigneau
University of Copenhagen, Department of Biology
The development of three-dimensional (3D) cell cultures has provided new tools for basic research and pre-clinical approaches, allowing the culture of cancer cells under conditions that closely resemble tumour growth in a living organism. We will provide an overview of the main 3D techniques used in our lab, including spheroids, cysts, organoids and microfluidics technology. We will discuss the advantages and limitations of the classical 3D models as well as recent advances in 3D culture techniques, focusing on how these culture methods have been used to study cancer progression.